Development of Liposomal Formulations of Zinc Diethyldithiocarbamate for Colorectal Cancer Treatment

(DDC)2Zn, disulfiram (DS) metabolite, has shown promisinganticancer effects in vitro but further investigations vivo are limited by itspoor water solubility. In this study, liposomes assessed as a deliverysystem for (DDC)2Zn. Liposomes were prepared the thin-filmhydration method, followed high-pressure homogenisation (HPH) sizereduction. The nano-liposomes then characterised size, polydispersityindex (PDI), zeta potential (ZP), drug loading and encapsulation efficiencies(DLE% EE%), MTT cytotoxicity assay. HSPC-based (PBS) liposomesshowed nano-range of sizes (< 200nm), good PDI (<0.5) moderate EE%(<40%). However, (DDC)2Zn liposomal formulations showed enhanced cytotoxicactivities toward colorectal cancer cells. Therefore, of(DDC)2Zn with improved DLE% EE% might have immense incancer therapy.